Pcr blunt ii topo manual transfer

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pcrbluntii topopcr4 blunt topo map

pcr-blunt ii-topo sequence

topo cloning

Add 1 µL of PCR reaction to 1 µL of TOPO cloning vector. 2. Incubate 5 min at II. Apa. I. pCR-Blunt II-. TOPO. TOPO. TOPO. lacZ?–ccdB. K a n a m yc in. Ampicilliin ways to clone a gene, but only Gateway technology lets you rapidly transfer.Alt Name: pCR-Blunt II-TOPO. Analyze: Sequence. Plasmid Type: Unspecified. Cloning Method: TOPO Cloning. Size: 3519. 5′ Sequencing 1 Primer: SP6.
Found that passive transfer of this antibody protected guinea pigs from lethal Ebola challenge. Amplified by RT-PCR the genes from Vero cells infected with Ebola Zaire (Mayinga) and cloned them into pCRO-Blunt II-TOPO vector.
Zero Blunt™ TOPO™ PCR Cloning Kit, with pCR™-Blunt II-TOPO™ Vector, One Shot™ TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid
the bluntended PCR products can be sub-cloned into pCRO-Blunt-II TOPO, artificial miRNAs is to maintain the structural and sequence recognition motifs
TOPO reactions! USER GUIDE. For Research Use Only. Not for use in diagnostic 2. ?85°C to ?68°C. Zero Blunt® TOPO®. PCR Cloning Kits for Sequencing arcing. 2. Transfer 2 µL of the diluted TOPO® Cloning reaction (from step 1 of this.
Can I use T-overhang TOPO cloning vector for blunt end PCR product? using the TOPO TA Cloning Kit by Invitrogen (with pCR 2.1 TOPO vector) and the After plating and ON incubation, single colonies is transferred to a small amount (10
Feb 24, 2014 –
The fragments obtained from the first PCR were mixed and amplified by PCR using mutations in ATPS2 were cloned into pCR-Blunt II-TOPO and fully sequenced. The binary plasmids were transferred to Agrobacterium tumefaciens C58C1 ACCESSION NUMBERS The reference sequence information on Arabidopsis
Jun 17, 2010 –

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