Red cell metabolism a manual of biochemical methods 1984

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Manual of Biochemical Methods, Grune and€ Red cell metabolism: a manual of biochemical methods – Ernest. In “Red Blood Cells of Domestic Mammals” N. S. Agar and P. G. Board, eds., pp. 133-164.
Ernest Beutler (September 30, 1928 – October 5, 2008) was a German-born American hematologist and biomedical scientist. He made important discoveries about the causes of a number of diseases, including anemias, Gaucher disease, disorders of iron metabolism and Tay-Sachs disease.He was also among the first scientists to identify X-inactivation as the genetic basis of tissue mosaicism in
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Simon LM, Fatrai Z, Jonas DE, Matkovics B. Study of metabolism enzymes during the development of Phaseolus vulgaris. Plant Physiol Biochem. 1974; 166:389-393. Beutler E. Red Cell Metabolism: A manual of biochemical methods. New York: Grune & Stratton; 1984. Goldberg DM, Spooner RJ. In: Methods of enzymatic analysis. Volume 111.
Summary. The effect of type I (insulin-dependent) diabetes mellitus on the key glycolytic enzymes of red cells was studied. The activities of hexokinase, phosphofructokinase and pyruvate kinase were found to be significantly (p<0.01) increased in diabetic patients.
Beutler E. Red cell metabolism: A manual of biochemical methods, Grune and Stratton, New York 1984. Grace RF, Zanella A, Neufeld EJ, et al. Erythrocyte pyruvate kinase deficiency: 2015 status report. Am J Hematol 2015; 90:825.
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Aspects of metabolism studied were the levels of ATP, 2,3-diphosphoglycerate (2,3-DPG) and reduced glutathione (GSH), activities of erythrocyte enzymes, glycolytic rate, the rate of glutathione S-conjugate transport (GCT), susceptibility of erythrocytes to an oxidising agent and the effect of eucalyptus oil on the red cells.
CHAPTER 26 ENERGY METABOLISM AND MAINTENANCE OF ERYTHROCYTES Williams Hematology CHAPTER 26 ENERGY METABOLISM AND MAINTENANCE OF ERYTHROCYTES ERNEST BEUTLER Glucose Metabolism Pathways Enzymes The Utilization of Substrates other than Glucose as Energy Sources Adenosine Fructose Mannose Galactose Dihydroxyacetone Glycogen Metabolism Glutathione Metabolism of the Erythrocyte Methemoglobin
(3rd edn.), Red Cell Metabolism, A Manual of Biochemical Methods, Grune and Stratton, New York (1984) Beutler and Matsumoto, 1975 E. Beutler, F. Matsumoto Ethinic variation in red cell glutathione peroxidase activity
Red cell metabolism: a manual of biochemical methods. Ernest Beutler. p-mercaptoethanol-EDTA stabilizing solution perchloric acid pyruvate kinase reagents are added recorder expansion giving red cells spectrophotometer standard deviation Red cell metabolism: a manual of biochemical methods:
Red cell metabolism: a manual of biochemical methods. Ernest Beutler. p-mercaptoethanol-EDTA stabilizing solution perchloric acid pyruvate kinase reagents are added recorder expansion giving red cells spectrophotometer standard deviation Red cell metabolism: a manual of biochemical methods:
Red cells were pelleted by centrifugation at 800 ? g for 10 min at 4 °C. After the removal of plasma (immediately frozen at ?80 °C until use for biochemical assays), buffy coat, and the upper 15% of packed RBC, the packed RBCs were washed twice with cold phosphate buffered saline (PBS) (0.9% NaCl and 10 mmoL/L Na 2 HPO 4 ; pH 7.4) and then
In: A Manual of Biochemical Methods, 3rd ed, Grune and Stratton, New York 1984. Motulsky AG, Yoshida A. Methods for the study of red cell, glucose-6-phosphate dehydrogenase. In: Biochemical Methods in Red Cell Genetics, Yunis JJ (Ed), Academic Press, New York 1969.
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